Research Article
Identification and Validation of Reference Genes for qRT-PCR Studies of Gene Expression in Dioscorea opposita
Table 1
Selected candidate reference genes and target genes for reference gene validation, primers, and amplicon characteristics.
| Gene symbol | Gene name | Accession number/RNA-Seq number | Primer sequence (5′-3′) | Amplicon length (bp) | (°C) | Amplification efficiency (%) | R2 |
| TUB | Beta-tubulin | comp80557_c0 | CTTCGTGTTTGGGCAGTCT | 116 | 60 | 101.205 0.019 | 0.979 | CACAGTTCTCGGCCTCCTT |
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | KR361321 | CAGCTCATTTGAAGGGTGG | 156 | 60 | 98.269 0.013 | 0.996 | CAAGAGGAGCAAGGCAGTT |
| ACT | Actin | KR361320 | CTCATTGATCGGCATGGAAGC | 130 | 60 | 95.536 0.007 | 0.998 | GGGGAACATAGTTGAACCACCAC |
| APT | Adenine phosphoribosyltransferase | comp78359_c0 | CAGGCAAAACAATTTCAGAAGC | 137 | 60 | 99.376 0.011 | 0.983 | AGCACGAAGTGTCCCACCA |
| MDH | Malate dehydrogenase | comp84254_c0 | GCCTTCCACAACCTTCAC | 224 | 60 | 96.463 0.012 | 0.994 | AGAATGGCAGCTCGTTCA |
| PP2A | Protein phosphatase 2A subunit A3 | comp74046_c0 | ATGCCAATGTCTGGAAGTA | 139 | 60 | 97.296 0.007 | 0.986 | CGGTCAAGAGCACGAAT |
| TIP41 | TIP41-like family protein | comp79289_c0 | TGTGCCAAATTCACCAA | 133 | 60 | 103.415 0.021 | 0.993 | CAAAACCACCTCATCATAGA |
| GUSB | Beta-glucuronidase | comp87528_c0 | GCCGAGCGGATGTAAGA | 141 | 60 | 98.396 0.016 | 0.973 | TGTTGTGAGTTGCCCTGT |
| EF1-α | Elongation factor 1-alpha | comp77681_c0 | ACTGTTCCTGTTGGTCGTG | 122 | 60 | 99.426 0.008 | 0.984 | TCTGGGAGGGATTCGTG |
| UBQ | Ubiquitin | comp74534_c0 | GGGCTTTCAAGGTCGTC | 100 | 60 | 99.378 0.009 | 0.971 | TGAAGGGTTTGCTCATCC |
| PE2.1 | Pectinesterase 2.1 | comp86916_c0 | GATCAACAGGTGCAACATCGAG | 116 | 60 | 98.395 0.019 | 0.966 | CGGCGTCACCAAAGATGAAG |
| PE53 | Pectinesterase 53 | comp76893_c0 | CTAAGGGATGGTATAACTGGGGAG | 175 | 60 | 99.397 0.016 | 0.991 | CAATCAGAGCCGTCAATGAAGC |
|
|
: correlation coefficient.
|