Identification and Validation of Reference Genes for qRT-PCR Studies of Gene Expression in Dioscorea opposita

Table 1

Selected candidate reference genes and target genes for reference gene validation, primers, and amplicon characteristics.

Gene symbol	Gene name	Accession number/RNA-Seq number	Primer sequence (5′-3′)	Amplicon; length (bp)	(°C)	Amplification; efficiency (%)	R^2
TUB	Beta-tubulin	comp80557_c0	CTTCGTGTTTGGGCAGTCT	116	60	101.205 0.019	0.979
			CACAGTTCTCGGCCTCCTT
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	KR361321	CAGCTCATTTGAAGGGTGG	156	60	98.269 0.013	0.996
			CAAGAGGAGCAAGGCAGTT
ACT	Actin	KR361320	CTCATTGATCGGCATGGAAGC	130	60	95.536 0.007	0.998
			GGGGAACATAGTTGAACCACCAC
APT	Adenine phosphoribosyltransferase	comp78359_c0	CAGGCAAAACAATTTCAGAAGC	137	60	99.376 0.011	0.983
			AGCACGAAGTGTCCCACCA
MDH	Malate dehydrogenase	comp84254_c0	GCCTTCCACAACCTTCAC	224	60	96.463 0.012	0.994
			AGAATGGCAGCTCGTTCA
PP2A	Protein phosphatase 2A; subunit A3	comp74046_c0	ATGCCAATGTCTGGAAGTA	139	60	97.296 0.007	0.986
			CGGTCAAGAGCACGAAT
TIP41	TIP41-like family protein	comp79289_c0	TGTGCCAAATTCACCAA	133	60	103.415 0.021	0.993
			CAAAACCACCTCATCATAGA
GUSB	Beta-glucuronidase	comp87528_c0	GCCGAGCGGATGTAAGA	141	60	98.396 0.016	0.973
			TGTTGTGAGTTGCCCTGT
EF1-α	Elongation factor 1-alpha	comp77681_c0	ACTGTTCCTGTTGGTCGTG	122	60	99.426 0.008	0.984
			TCTGGGAGGGATTCGTG
UBQ	Ubiquitin	comp74534_c0	GGGCTTTCAAGGTCGTC	100	60	99.378 0.009	0.971
			TGAAGGGTTTGCTCATCC
PE2.1	Pectinesterase 2.1	comp86916_c0	GATCAACAGGTGCAACATCGAG	116	60	98.395 0.019	0.966
			CGGCGTCACCAAAGATGAAG
PE53	Pectinesterase 53	comp76893_c0	CTAAGGGATGGTATAACTGGGGAG	175	60	99.397 0.016	0.991
			CAATCAGAGCCGTCAATGAAGC

: correlation coefficient.