Development of a Rapid Point-of-Use DNA Test for the Screening of Genuity® Roundup Ready 2 Yield® Soybean in Seed Samples

Figure 1

Target specific amplification of a DNA sequence using RPA. (a) Schematic of PCR and RPA processes. RPA uses recombinases and polymerase to bind and elongate the primers at a constant temperature resulting in the duplication of the target sequence. Multiple events of such duplication lead to exponential amplification under isothermal condition. On the other hand, PCR relies on thermal cycling and heat-stable polymerase for the amplification of the target sequence. (b) Organization of sequences at the site of RR2Y insertion in the conventional and RR2Y soybean genomes. (c) Arrangement of primers and probe used for RPA mediated amplification and detection of the RR2Y specific insertion.

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