Development of a Rapid Point-of-Use DNA Test for the Screening of Genuity® Roundup Ready 2 Yield® Soybean in Seed Samples

Figure 5

RPA-based field detection method. (a) Protein-based lateral-flow strips were used to detect conventional, RR1, and RR2Y yield soybean extracts. Genetic modification in the RR1 and RR2Y events produces the unique CP4 EPSPS protein not observed in the conventional soybean. (b) Flow chart depicting the sample processing and assay setup steps to perform the protein-based and RPA-based field detection methods. (c) Specificity and sensitivity of RPA-based RR2Y event specific amplification using crude seed extracts as the templates. Endogenous lec gene is also amplified in the duplex reaction as a control. (d) RR2Y soybean specific amplification reactions performed by trained (left) and untrained (middle and right) personnel to demonstrate the ease of carrying out RPA-based field detection assays.

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