Research Article
Development of a Rapid Point-of-Use DNA Test for the Screening of Genuity® Roundup Ready 2 Yield® Soybean in Seed Samples
Table 1
Sequences of RPA and PCR primers and probes used in the study.
| Assay | Description | Sequence |
| RPA | RR2Y probe | cccgccttcagtttaaactatcagtgtttggagc-T(BHQ-2)-t-dSpacer-a-T(TAMRA)-aaccacgattgaag | RR2Y forward primer | ccctcttggcttttctaagtttgagctcgttactg | RR2Y reverse primer | cccgccttcagtttaaactatcagtgtttgg | lec probe | ggaaactgtttctttcagctggaacaag-T(FAM)-t-dSpacer-g-T(BHQ-1)-gccgaagcaacc | lec forward primer | ccagaatgtggttgtatctctctccctaacctt | lec
reverse primer | cccgaggaggtcacaatagcgtctccttggag |
| PCR | RR1 forward primer | tttgggaccactgtcggcagaggcatctt | RR1 reverse primer | gatttgaattcagaaccttgtgca | RR2Y forward primer | tcccgctctagcgcttcaat | RR2Y reverse primer | tcgagcaggacctgcagaa | lec forward primer | gtttgacactttccggaactcttg | lec reverse primer | ctgtcacatttagatggcctcatg |
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BHQ = dT Black Hole Quencher; TAMRA = dT TAMRA; FAM = dT FAM.
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