Phytochemical Screening and Antinociceptive and Antidiarrheal Activities of Hydromethanol and Petroleum Benzene Extract of Microcos paniculata Barks

Table 5

Effect of standard, HMPB, and PBMPB in hot plate test.

Group	Dose	Response latency period (s)
		0 min	+30 min	+60 min	+120 min	+180 min
Control	10 mL/kg	2.23 ± 0.02	2.24 ± 0.01	2.19 ± 0.01	2.21 ± 0.01	2.25 ± 0.00
Tramadol	10 mg/kg	2.34 ± 0.01	5.25 ± 0.01	6.65 ± 0.02	6.46 ± 0.01	3.53 ± 0.13
HMPB	200 mg/kg	8.15 ± 0.01	8.73 ± 0.01	5.84 ± 0.01	6.39 ± 0.01	6.65 ± 0.01
HMPB	400 mg/kg	6.18 ± 0.01	8.08 ± 0.01	7.35 ± 0.04	7.21 ± 0.00	8.75 ± 0.01
PBMPB	200 mg/kg	7.09 ± 0.03	7.72 ± 0.05	3.32 ± 0.01	3.86 ± 0.01	4.54 ± 0.01
PBMPB	400 mg/kg	9.46 ± 0.02	7.20 ± 0.03	4.66 ± 0.01	5.34 ± 0.01	2.98 ± 0.00

Response latency values are presented as mean ± standard error. mice in each group. 0 min means 30 min before drug administration; +30 min, +60 min, +120 min, and +180 min indicate 30, 60, 120, and 180 min after drug administration, respectively. ⁢, versus control (Dunnett’s -test); , versus tramadol 10 mg/kg; , versus HMPB 200 mg/kg; , versus HMPB 400 mg/kg; , versus PBMPB 200 mg/kg; , versus PBMPB 400 mg/kg (pairwise comparison by post hoc Tukey’s HSD test).
Tests of within-subjects effects conducted by repeated measure analysis of variance reveal that for the factor “time” calculated for all methods and value = 0.000 in every case. So time is highly significant at any level of significance.