Isolation and Physicochemical Characterization of Laccase from Ganoderma lucidum-CDBT1 Isolated from Its Native Habitat in Nepal

Figure 5

pH and temperature optimum and pH and thermal stability of purified laccase. The experimental conditions were as described in Materials and Methods. Experiments described in (a) and (c) were performed at room temperature (~25°C). The experimental results depicted in (a) and (c) (pH optimum and pH stability) were performed using buffers of various pH specified, whereas the temperature optimum and thermal stability was performed at pH 5 (0.1 M sodium acetate, pH 5.0).

(a) pH optimum

(b) Temperature optimum

(c) pH stability

(d) Temperature stability