Improved Production of Aspergillus usamii endo-β-1,4-Xylanase in Pichia pastoris via Combined Strategies

Table 1

Comparison of the codon usage for native (xynB) and synthetic (xynB-opt) targeted genes  at Pichia pastoris for expression.

AA	Codon	Host fraction	xynB	xynB-opt
Gly	GGG	0.10	0	0
	GGA	0.32	9	11
	GGT	0.44	5	18
	GGC	0.14	15	0
Glu	GAG	0.43	6	6
	GAA	0.57	0	0
Asp	GAT	0.58	3	2
	GAC	0.42	7	8
Val	GTG	0.19	3	0
	GTA	0.15	0	0
	GTT	0.42	6	1
	GTC	0.23	6	14
Ala	GCG	0.06	2	0
	GCA	0.23	0	0
	GCT	0.45	12	7
	GCC	0.26	4	11
Arg	AGG	0.16	0	0
	AGA	0.48	1	2
	CGG	0.05	0	0
	CGA	0.10	0	0
	CGT	0.16	2	3
	CGC	0.05	2	0
Lys	AAG	0.53	6	5
	AAA	0.47	0	1
Ser	AGT	0.15	3	0
	AGC	0.09	4	0
	TCG	0.09	5	0
	TCA	0.19	0	0
	TCT	0.29	3	6
	TCC	0.20	9	18
Stop	TAA	0.53	1	1
Asn	AAT	0.49	2	0
	AAC	0.51	13	15
Met	ATG	1.00	3	3
Ile	ATA	0.19	0	0
	ATT	0.50	1	1
	ATC	0.30	6	6
Thr	ACG	0.11	1	0
	ACA	0.24	1	0
	ACT	0.40	4	4
	ACC	0.25	19	23
Trp	TGG	1.00	6	6
Cys	TGT	0.65	0	1
	TGC	0.35	1	0
Tyr	TAT	0.46	2	1
	TAC	0.55	15	16
Leu	TTG	0.33	1	8
	TTA	0.16	0	0
	CTG	0.16	4	0
	CTA	0.11	0	0
	CTT	0.16	1	3
	CTC	0.08	5	0
Phe	TTT	0.54	2	1
	TTC	0.46	5	6
Gln	CAG	0.39	8	7
	CAA	0.61	0	1
His	CAT	0.57	0	0
	CAC	0.43	4	4
Pro	CCC	0.15	4	0
	CCG	0.09	1	0
	CCA	0.41	0	6
	CCT	0.35	1	0