iTRAQ-Based Proteomics Identification of Serum Biomarkers of Two Chronic Hepatitis B Subtypes Diagnosed by Traditional Chinese Medicine

Table 4

Up- and downregulated serum proteins exclusively in DHSM-CHB.

UniProt_ID	Gene_name	Description	Number of significant matches	Number of significant sequences	emPAI
Upregulated
A0A024R145_HUMAN	ALDOB	Fructose-bisphosphate aldolase	5	3	0.22	1.29
A0A024R1N1_HUMAN	MYH9	Myosin, heavy polypeptide 9, nonmuscle, isoform CRA_a	17	9	0.13	1.43
A0A024R5H8_HUMAN	RAB6A	RAB6A, member RAS oncogene family, isoform CRA_b	8	3	0.57	1.43
A0A024R694_HUMAN	ACTN1	Actinin, alpha 1, isoform CRA_a	20	10	0.42	1.33
A0A024R6G3_HUMAN	FBLN5	Fibulin 5, isoform CRA_b	6	3	0.27	1.33
A0A024R9T1_HUMAN	hCG_39634	HCG39634, isoform CRA_a	2	2	0.26	1.49
A0A024RDB8_HUMAN	HPSE	Heparanase, isoform CRA_a	23	6	0.55	1.36
A0A024RDL8_HUMAN	ASL	Argininosuccinate lyase isoform 1	10	5	0.39	1.65
A0A087WT59_HUMAN	TTR	Transthyretin	175	7	5.66	1.34
A0A087WUL0_HUMAN	TKFC	Triokinase/FMN cyclase	3	3	0.15	1.28
A0A0A0MSD0_HUMAN	SVEP1	Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1	4	3	0.02	1.36
A0A0A0MT32_HUMAN	LIPA	Lysosomal acid lipase/cholesteryl ester hydrolase	2	1	0.09	1.38
A0A0C4DFP6_HUMAN	CRTAC1	Cartilage acidic protein 1	6	5	0.24	1.23
A0A0S2Z3F6_HUMAN	CETP	Cholesteryl ester transfer protein plasma isoform 1 (fragment)	95	14	3.56	1.36
A0A125QYY9_HUMAN		IBM-B2 heavy chain variable region (fragment)	12	2	0.83	1.42
A0N7I9_HUMAN	F5-20	F5-20 (fragment)	4	1	0.47	1.68
A2NYU9_HUMAN		Heavy chain Fab (fragment)	2	1	0.48	1.29
A8K486_HUMAN		Peptidyl-prolyl cis-trans isomerase	2	2	0.32	1.40
ADIPO_HUMAN	ADIPOQ	Adiponectin	27	5	2.13	1.32
APOF_HUMAN	APOF	Apolipoprotein F	7	3	0.5	1.29
ASSY_HUMAN	ASS1	Argininosuccinate synthase	4	3	0.18	1.49
ATPB_HUMAN	ATP5B	ATP synthase subunit beta, mitochondrial	11	2	0.22	1.34
B3KTV0_HUMAN		cDNA FLJ38781 fis, clone LIVER2000216, highly similar to HEAT SHOCK COGNATE 71 kDa PROTEIN	5	5	0.21	1.29
B4DQK4_HUMAN		cDNA FLJ53743, highly similar to tubulin alpha-3 chain	21	6	0.81	1.61
B4DVA7_HUMAN		Beta-hexosaminidase	3	2	0.15	1.26
B7Z478_HUMAN	PSMB2	Proteasome (prosome, macropain) subunit, beta type, 2, isoform CRA_b	2	1	0.3	1.65
BPIB1_HUMAN	BPIFB1	BPI fold-containing family B member 1	11	6	0.51	1.28
BTD_HUMAN	BTD	Biotinidase	47	6	1.12	1.54
CAMP_HUMAN	CAMP	Cathelicidin antimicrobial peptide	2	1	0.29	1.43
CAND1_HUMAN	CAND1	Cullin-associated NEDD8-dissociated protein 1	2	1	0.02	1.36
CATD_HUMAN	CTSD	Cathepsin D	2	1	0.13	1.28
DHSO_HUMAN	SORD	Sorbitol dehydrogenase	4	3	0.32	1.67
FIBA_HUMAN	FGA	Fibrinogen alpha chain	102	22	2.36	1.36
G3P_HUMAN	GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	19	7	1.37	1.37
G3V5Z7_HUMAN	PSMA6	Proteasome subunit alpha type	7	4	0.58	1.44
HV310_HUMAN		Ig heavy chain V-III region HIL	3	1	0.5	1.48
HV320_HUMAN		Ig heavy chain V-III region GAL	36	3	1.91	1.32
KV404_HUMAN		Ig kappa chain V-IV region B17	34	2	1.45	1.45
LDHB_HUMAN	LDHB	L-lactate dehydrogenase B chain	4	2	0.33	1.65
M0QZB5_HUMAN	PPFIA4	Liprin-alpha-4 (fragment)	2	1	0.03	1.33
MARCO_HUMAN	MARCO	Macrophage receptor MARCO	2	1	0.11	1.31
MDR3_HUMAN	ABCB4	Phosphatidylcholine translocator ABCB4	2	1	0.04	1.32
OTUB1_HUMAN	OTUB1	Ubiquitin thioesterase OTUB1	2	1	0.09	1.27
PIGR_HUMAN	PIGR	Polymeric immunoglobulin receptor	7	4	0.18	1.34
PSA3_HUMAN	PSMA3	Proteasome subunit alpha type-3	8	4	0.58	1.31
PSB1_HUMAN	PSMB1	Proteasome subunit beta type-1	4	3	0.5	1.47
Q6GMX4_HUMAN	IGL@	IGL@ protein	442	6	7.15	1.27
Q6PIQ7_HUMAN	IGL@	IGL@ protein	441	6	6.39	1.27
Q6ZNX5_HUMAN		CDNA FLJ26936 fis, clone RCT06808	5	1	0.39	1.78
Q9UL82_HUMAN		Myosin-reactive immunoglobulin light chain variable region (fragment)	46	3	2.11	1.41
QSOX1_HUMAN	QSOX1	Sulfhydryl oxidase 1	97	20	2.33	1.24
TBA4A_HUMAN	TUBA4A	Tubulin alpha-4A chain	24	8	0.86	1.55
TBB1_HUMAN	TUBB1	Tubulin beta-1 chain	6	4	0.33	1.30
TBB8_HUMAN	TUBB8	Tubulin beta-8 chain	7	5	0.42	1.32
TRML1_HUMAN	TREML1	Trem-like transcript 1 protein	3	1	0.09	1.42
TSP1_HUMAN	THBS1	Thrombospondin-1	365	34	4.23	1.37
TYPH_HUMAN	TYMP	Thymidine phosphorylase	3	3	0.19	1.23
Downregulated
A0A024CIM4_HUMAN		Carboxylic ester hydrolase	7	3	0.17	0.81
A0A024R6I6_HUMAN	SERPINA10	Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10, isoform CRA_b	65	12	2.73	0.75
A0A024R6K8_HUMAN	WARS	Tryptophanyl-tRNA synthetase, isoform CRA_a	5	2	0.1	0.82
A0A024R853_HUMAN	IQCE	IQ motif containing E, isoform CRA_b	2	1	0.04	0.74
A0A024R9J3_HUMAN	COLEC10	Collectin subfamily member 10 (C-type lectin), isoform CRA_a	4	2	0.28	0.83
A0A075B6S2_HUMAN	IGKV2D-29	Protein IGKV2D-29 (fragment)	26	3	2.47	0.77
A0A087X054_HUMAN	HYOU1	Hypoxia upregulated protein 1	4	2	0.08	0.58
A0A0A7C3P2_HUMAN	HLA-A	MHC class I antigen (fragment)	8	3	0.72	0.77
A0A0J9YX35_HUMAN		Uncharacterized protein (fragment)	2	1	0.51	0.74
A0A0X9TD47_HUMAN		MS-D1 light chain variable region (fragment)	30	3	5.49	0.83
A2AP_HUMAN	SERPINF2	Alpha-2-antiplasmin	67	10	1.86	0.74
A2J1N4_HUMAN		Rheumatoid factor RF-IP24 (fragment)	10	2	1.08	0.80
A2J1N5_HUMAN		Rheumatoid factor RF-ET6 (fragment)	15	1	1.65	0.79
A2MYD0_HUMAN	V1-17	V1-17 protein (fragment)	8	3	1.87	0.83
A2NB45_HUMAN		Cold agglutinin FS-1 L-chain (fragment)	23	2	1.39	0.69
A3RKG7_HUMAN		Coagulation factor VII (fragment)	17	6	2.02	0.77
ALBU_HUMAN	ALB	Serum albumin	1841	31	17.82	0.67
AMBP_HUMAN	AMBP	Protein AMBP	34	3	0.75	0.76
ATS13_HUMAN	ADAMTS13	A disintegrin and metalloproteinase with thrombospondin motifs 13	22	8	0.26	0.81
B2RBZ5_HUMAN		cDNA, FLJ95778, highly similar to Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10 (SERPINA10), mRNA	58	11	2.2	0.76
B4DL32_HUMAN		cDNA FLJ59922, highly similar to keratin, type II cytoskeletal 5	7	2	0.33	0.83
B4DN21_HUMAN		cDNA FLJ53365, highly similar to Homo sapiens fibronectin 1 (FN1), transcript variant 4, mRNA	384	8	6.85	0.77
B4E1B2_HUMAN		cDNA FLJ53691, highly similar to serotransferrin	142	16	2.78	0.59
B7Z8B6_HUMAN		cDNA FLJ54395, highly similar to inter-alpha-trypsin inhibitor heavy chain H1	68	8	1.2	0.71
BIG3_HUMAN	ARFGEF3	Brefeldin A-inhibited guanine nucleotide-exchange protein 3	3	1	0.01	0.71
BLVRB_HUMAN	BLVRB	Flavin reductase (NADPH)	6	2	0.28	0.81
CATA_HUMAN	CAT	Catalase	3	3	0.15	0.80
CATF_HUMAN	CTSF	Cathepsin F	6	4	0.23	0.81
CO2_HUMAN	C2	Complement C2	20	9	0.5	0.78
CO5_HUMAN	C5	Complement C5	416	52	4.64	0.74
F13B_HUMAN	F13B	Coagulation factor XIII B chain	4	3	0.15	0.76
GLUC_HUMAN	GCG	Glucagon	2	1	0.3	0.81
H0YLF3_HUMAN	B2M	Beta-2-microglobulin (fragment)	4	1	0.75	0.77
HEMO_HUMAN	HPX	Hemopexin	63	9	1.51	0.65
HGFA_HUMAN	HGFAC	Hepatocyte growth factor activator	15	4	0.39	0.82
HV306_HUMAN		Ig heavy chain V-III region BUT	36	5	6.45	0.76
HV314_HUMAN		Ig heavy chain V-III region LAY	3	2	0.88	0.81
HV317_HUMAN		Ig heavy chain V-III region ZAP	3	1	0.56	0.78
IC1_HUMAN	SERPING1	Plasma protease C1 inhibitor	34	7	0.82	0.62
INHBC_HUMAN	INHBC	Inhibin beta C chain	16	3	0.36	0.82
ITIH1_HUMAN	ITIH1	Inter-alpha-trypsin inhibitor heavy chain H1	252	22	3	0.81
K1C10_HUMAN	KRT10	Keratin, type I cytoskeletal 10	32	8	0.87	0.70
K1C14_HUMAN	KRT14	Keratin, type I cytoskeletal 14	3	2	0.11	0.68
K1C9_HUMAN	KRT9	Keratin, type I cytoskeletal 9	24	8	0.65	0.81
K22E_HUMAN	KRT2	Keratin, type II cytoskeletal 2 epidermal	38	14	1.31	0.77
K2C1_HUMAN	KRT1	Keratin, type II cytoskeletal 1	104	19	4.31	0.66
K2C6B_HUMAN	KRT6B	Keratin, type II cytoskeletal 6B	18	4	0.51	0.63
K7ER74_HUMAN	APOC4-APOC2	Protein APOC4-APOC2	111	5	7.56	0.68
KV201_HUMAN		Ig kappa chain V-II region Cum	23	2	1.98	0.54
L0R8K6_HUMAN	TRIM25	Alternative protein TRIM25	13	1	0.9	0.77
MASP1_HUMAN	MASP1	Mannan-binding lectin serine protease 1	36	7	0.63	0.83
PCDGG_HUMAN	PCDHGB4	Protocadherin gamma-B4	3	1	0.03	0.80
PLA1A_HUMAN	PLA1A	Phospholipase A1 member A	2	1	0.07	0.83
PROC_HUMAN	PROC	Vitamin K-dependent protein C	84	9	1.71	0.78
Q06AH7_HUMAN	TF	Transferrin	141	16	2.64	0.59
Q1T720_HUMAN	HLA-B	MHC class I antigen (fragment)	9	6	0.9	0.77
Q6U2L6_HUMAN	C4B	C4B (fragment)	79	6	27.02	0.69
Q9P1C5_HUMAN		PRO2769	43	7	0.97	0.70
Q9UMV1_HUMAN	C4B	Complement C4B1a (fragment)	8	1	1.56	0.77
Q9UNU2_HUMAN	C4B	Complement protein C4B frameshift mutant (fragment)	524	14	26.83	0.64
QPCT_HUMAN	QPCT	Glutaminyl-peptide cyclotransferase	7	4	0.43	0.83
TIMP3_HUMAN	TIMP3	Metalloproteinase inhibitor 3	2	1	0.11	0.75

change provided by MASCOT.
values calculated by edgeR to show the significance of different expression.