Resistin Enhances Inflammatory Cytokine Production in Coronary Artery Tissues by Activating the NF-κB Signaling

Figure 3

Quantitative analysis of the levels of TNF-α, MMP-9, and IL-1β expression in the heart tissues of mice. C57BL/6 mice were injected with LCWE (0.5 mg) to induce coronary arteritis and the levels of TNF-α, MMP-9, and IL-1β expression in the hearts of individual groups of mice at the indicated time points after induction were determined by quantitative RT-PCR and ELISA. Data are expressed as the mean ± SD of each group ( per group per time point) of mice from three separate experiments. (a–c) RT-PCR analysis of the TNF-α, MMP-9, and IL-1β mRNA transcripts. (d–f) ELISA analysis of the levels of TNF-α (×10² μg/mL), MMP-9 (×10³ μg/mL), and IL-1β (×10³ μg/mL) proteins. versus the LCWE group.

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