Research Article

Skin Treatment with Pulsed Monochromatic UVA1 355 Device and Computerized Morphometric Analysis of Histochemically Identified Langerhans Cells

Figure 1

More relevant steps of the image analysis procedure illustrated on a skin control sample. (a) Immunohistochemical analysis identified CD1a-positive Langerhans cells (brown) on a background of keratinocytes faintly hematoxylin stained. (b) Continuous tone image with labeled CD1a-positive Langerhans cells, blue masked following segmentation (inset), ready for digital conversion. (c) Binary image resulting from digitalization where white areas are constituting the pool of pixels to be evaluated.
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