Expression and Purification of C-Peptide Containing Insulin Using Pichia pastoris Expression System
(a) Schematic diagram showing construction of recombinant pPICZ-α-proinsulin plasmid. (b) Agarose gel electrophoresis showing PCR amplification of proinsulin cDNA. Lane 1: DNA marker; lanes 2–5: PCR amplified proinsulin cDNA.
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