BioMed Research International / 2016 / Article / Fig 2

Research Article

Expression and Purification of C-Peptide Containing Insulin Using Pichia pastoris Expression System

Figure 2

(a) Agarose gel electrophoresis for screening of positive E. coli clones harboring recombinant pPICZ-α-proinsulin. Lanes 1 and 14: DNA marker; lanes 2 to 13 and 15 to 26: plasmid DNA isolated from Zeocin resistant E. coli transformants and double-digested with EcoRI and XhoI. (b) Agarose gel electrophoresis for confirmation of clones by PCR. Lane 1: DNA marker; lanes 2–5: positive clones 1 to 4.

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