BioMed Research International / 2016 / Article / Fig 2

Research Article

Expression and Purification of C-Peptide Containing Insulin Using Pichia pastoris Expression System

Figure 2

(a) Agarose gel electrophoresis for screening of positive E. coli clones harboring recombinant pPICZ-α-proinsulin. Lanes 1 and 14: DNA marker; lanes 2 to 13 and 15 to 26: plasmid DNA isolated from Zeocin resistant E. coli transformants and double-digested with EcoRI and XhoI. (b) Agarose gel electrophoresis for confirmation of clones by PCR. Lane 1: DNA marker; lanes 2–5: positive clones 1 to 4.
(a)
(b)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.