Expression and Purification of C-Peptide Containing Insulin Using<i> Pichia pastoris</i> Expression System

<div>(a) Agarose gel electrophoresis for screening of positive<i> E. coli</i> clones harboring recombinant pPICZ-<i>α</i>-proinsulin. Lanes 1 and 14: DNA marker; lanes 2 to 13 and 15 to 26: plasmid DNA isolated from Zeocin resistant<i> E. coli</i> transformants and double-digested with<i> Eco</i>RI and<i> Xho</i>I. (b) Agarose gel electrophoresis for confirmation of clones by PCR. Lane 1: DNA marker; lanes 2–5: positive clones 1 to 4.</div>

BioMed Research International

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Figure 2: Expression and Purification of C-Peptide Containing Insulin Using<i> Pichia pastoris</i> Expression System 

BioMed Research International

Expression and Purification of C-Peptide Containing Insulin Using Pichia pastoris Expression System

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