Expression and Purification of C-Peptide Containing Insulin Using Pichia pastoris Expression System

Figure 3

Agarose gel electrophoresis for checking the integration of proinsulin cDNA into Pichia genome. Transformation of pPICZ-C-insulin into Pichia pastoris SuperMan₅ strain was done by electroporation and confirmation for integration of insulin cDNA in Pichia genome was done by PCR. Lanes 1 to 10: recombinant Pichia clones harboring pPICZ-α-proinsulin plasmid.