Placenta Derived Mesenchymal Stem Cells Hosted on RKKP Glass-Ceramic: A Tissue Engineering Strategy for Bone Regenerative Medicine Applications

Table 1

Sequence of primers used for qRT-PCR.

Target gene	Primer sequence	Annealing; temperature (C°)
VEGF	5′-cttgggtgcattggagcct-3′; 5′-ctgcgctgatagacatccat-3′	60
β-ACT	5′-gctcctcctgagcgcaag-3′; 5′catctgctggaaggtggaca-3′	60
Ki67	5′-tgaacaaaaggcaaagaagac-3′; 5′-gagctttccctattattatggt-3′	60
IDO	5′-tgctaaaggcgctgttggaa-3′; 5′-tacaccagaccgtctgatag-3′	60
HGF	5′-caatagcatgtcaagtggag-3′; 5′-ctgtgttcgtgtggtatcat-3′	60
TGF β1	5′-tcaagttaaaagtggagcagc-3′; 5′-actccggtgacatcaaaaga-3′	60
RPL34	5′-gaaacatgtcagcagggcc-3′; 5′-tgactctgtgcttgtgcctt-3′	60
RUNX2	5′-catcatctctgccccctct-3′; 5′-actcttgcctcgtccactc-3′	60
ALP	5′-caatgagggcaccgtggg-3′; 5′-tcgtggtggtcacaatgcc-3′	60
OCL	5′-gcagcgaggtagtgaagag-3′; 5′-gaaagccgatgtggtcagc-3′	60
GAPDH	5′-catcatctctgccccctct-3′; 5′-caaagttgtcatggatgacct-3′	60