Comparative Genomic Analysis of Delftia tsuruhatensis MTQ3 and the Identification of Functional NRPS Genes for Siderophore Production

Figure 5

Knockout target genes with the Km cassette. The target genes orf-1941, orf-1945, and orf-1946 were amplified from the genomic DNA of MTQ3, after a series of enzyme digestions and ligation to form the recombinant plasmids pK-1941, pK-1945, and pK-1946. Triparental mating was used to generate homologous recombinant strains.