Comparative Genomic Analysis of Delftia tsuruhatensis MTQ3 and the Identification of Functional NRPS Genes for Siderophore Production

Table 1

Bacterial strains and plasmids used in this work.

Strain/plasmid	Relevant characteristics	Source
D. tsuruhatensis
MTQ3	Wild-type strain, ,	[11]
MTQ3-Δ1941	D. tsuruhatensis MTQ3-Δ1941, ,	This work
MTQ3-Δ1945	D. tsuruhatensis MTQ3-Δ1945, ,	This work
MTQ3-Δ1946	D. tsuruhatensis MTQ3-Δ1946, ,	This work
E. coli
DH5α	Host of recombinant plasmids	TransGen
Plasmids
pJQ200SK	Suicide plasmid carrying sacB, , selecting double crossover MTQ3	[12]
pk-1941	pJQ200SK carrying Km and the fragment of orf-1941, ,	This work
pk-1945	pJQ200SK carrying Km and the fragment of orf-1945, ,	This work
pk-1946	pJQ200SK carrying Km and the fragment of orf-1946, ,	This work
pUC4K	Carrying Km cassette (Pst I),	[13]
pRK2013	Helper plasmid used in triparental mating, ,	[14]
pGEM-T easy	TA cloning vector,	Promega