Fecal Colonization with Extended-Spectrum Beta-Lactamase and AmpC-Producing Escherichia coli

Table 1

Primers used for amplification of the tested -lactamase genes (Dallenne et al., 2010 [8]).

PCR type	Target	Primer	Sequence of primers (5′-3′)	Amplified products (bp)
Multiplex I TEM, SHV, and OXA-1-like	TEM	MultiTSO-T_for	CATTTCCGTGTCGCCCTTATTC	800
		MultiTSO-T_rev	CGTTCATCCATAGTTGCCTGAC
	SHV	MultiTSO-S_for	AGCCGCTTGAGCAAATTAAAC	713
		MultiTSO-S_rev	ATCCCGCAGATAAATCACCAC
	OXA-1	MultiTSO-O_for	GGCACCAGATTCAACTTTCAAG	564
		MultiTSO-O_rev	GACCCCAAGTTTCCTGTAAGTG
Multiplex II CTX-M group 1, group 2, and group 9	CTX-M group 1	MultiCTXMGp1_for	TTAGGAARTGTGCCGCTGYA	688
		MultiCTXMGp1-2_rev	CGATATCGTTGGTGGTRCCAT
	CTX-M group 2	MultiCTXMGp2_for	CGTTAACGGCACGATGAC	404
		MultiCTXMGp1-2_rev	CGATATCGTTGGTGGTRCCAT
	CTX-M group 9	MultiCTXMGp9_for	TCAAGCCTGCCGATCTGGT	561
		MultiCTXMGp9_rev	TGATTCTCGCCGCTGAAG
CTX-M group 8/25	CTX-M group 8/25	CTX-Mg8/25_for	AACRCRCAGACGCTCTAC	326
		CTX-Mg8/25_rev	TCGAGCCGGAASGTGTYAT
Multiplex III ACC, FOX, MOX, DHA, CIT, and EBC	ACC-1 and ACC-2	MultiCaseACC_for	CACCTCCAGCGACTTGTTAC	346
		MultiCaseACC_rev	GTTAGCCAGCATCACGATCC
	FOX-1 to FOX-5	MultiCaseFOX_for	CTACAGTGCGGGTGGTTT	162
		MultiCaseFOX_rev	CTATTTGCGGCCAGGTGA
	MOX-1, MOX-2, CMY-1, CMY-8 to CMY-11, and CMY-19	MultiCaseMOX_for	GCAACAACGACAATCCATCCT	895
		MultiCaseMOX_rev	GGGATAGGCGTAACTCTCCCAA
	DHA-1 and DHA-2	MultiCaseDHA_for	TGATGGCACAGCAGGATATTC	997
		MultiCaseDHA_rev	GCTTTGACTCTTTCGGTATTCG
	LAT-1 to LAT-3, BIL-1, CMY-2 to CMY-7, CMY-12 to CMY-18, and CMY-21 to CMY-23	MultiCaseCIT_for	CGAAGAGGCAATGACCAGAC	538
		MultiCaseCIT_rev	ACGGACAGGGTTAGGATAGY
	ACT-1 and MIR-1	MultiCaseEBC_for	CGGTAAAGCCGATGTTGCG	683