A De-O-acylated Lipooligosaccharide-Based Adjuvant System Promotes Antibody and Th1-Type Immune Responses to H1N1 Pandemic Influenza Vaccine in Mice

Figure 3

CIA06-adjuvanted influenza vaccine stimulates both CD4⁺ and CD8⁺ T cell responses. Splenocytes were isolated from the mice () that had been immunized twice at a 3-week interval with nonadjuvanted or adjuvanted Greenflu-S (0.2 μg) and stimulated with the vaccine for 72 h in the absence or presence of anti-CD4 and/or anti-CD8 antibodies. IFN- levels in the culture media were determined by sandwich ELISA. Statistical differences were analyzed by one-way ANOVA followed by Tukey’s multiple comparison test. Results are expressed as the means ± SD of values obtained from triplicate cultures that used two spleens each. Data shown are representative of three independent experiments. ; ; and . NS, not significant.