Effect of miR-146a/bFGF/PEG-PEI Nanoparticles on Inflammation Response and Tissue Regeneration of Human Dental Pulp Cells

Figure 1

Synthesis and characteristics of PEG-PEI. (a) Nuclear magnetic resonance graph of the products: (A) mPEG-CDI, (B) mPEG-PEI. Strong characteristic peaks of PEG and PEI were shown to reside at about 3.65 ppm (peak b) and 7.35 ppm (peaks d and e), respectively. (b) Dynamic diameter distribution showed that miR-146a/PEG-PEI nanoparticles were in the range 150–360 nm at N/P ratio 1.5 to 40. The particle diameter of the nanoparticles was the lowest at the N/P ratio of 2 and maintained consistent upregulation along with the increasing of N/P value. (c) The zeta potentials were measured to 0 mV at the N/P ratio of 2 and upregulated with the increasing of N/P value. (d) Agarose gel electrophoresis image revealed that the electrophoretic mobility was partially neutralized and the bands were weakened with the increase of N/P value. The motion was completely retarded at N/P ratio of 2.

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