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BioMed Research International
Volume 2016, Article ID 4013487, 8 pages
http://dx.doi.org/10.1155/2016/4013487
Research Article

MicroRNA-146a Contributes to SCI Recovery via Regulating TRAF6 and IRAK1 Expression

1Department of Orthopedics, The First Affiliated Hospital of Jinan University, Guangzhou, China
2Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China
3Institute of Neurology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China
4Stroke Center, Neurology & Neurosurgery Division, The Clinical Medicine Research Institute and The First Affiliated Hospital of Jinan University, Guangzhou, China

Received 1 July 2016; Revised 27 July 2016; Accepted 11 August 2016

Academic Editor: Hai-Feng Pan

Copyright © 2016 Jinsong Wei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

MicroRNA-146a participates in spinal cord injury (SCI) recovery. Until recently, how miRNA-146a participates in SCI remained unclear. In this study, we tried to explore the roles of miRNA-146a in the recovery of SCI using a rat model. The expression of the probable target genes of miRNA-146a (including IRAK1 and TARF6) as well as proinflammation cytokines were measured until 7 days after surgery in the three groups (sham group, SCI group, and miRNA-146a antagomir injection group). Also, the animals’ motivations were estimated using Basso Beattie Bresnahan (BBB) during the whole experiment. A luciferase assay was performed to demonstrate that miRNA-146a could directly target the mRNAs of IRAK1 and TRAF6. Our experiments indicate that miRNA-146a inhibits proinflammatory cytokine secretion by suppressing IRAK1 and TRAF6 expression in the SCI model. In contrast, miRNA-146a may be upregulated by inflammatory mediators via the IRAK1/TRAF6 pathway in the spinal cord. As a negative feedback element, miRNA-146a could make sure that the expression of IRAK1- and TRAF6-mediated genes was under tight control. Thus, miRNA-146a may serve as a novel therapeutic target for SCI interventions.