The Functional Haplotypes of CHRM3 Modulate mRNA Expression and Associate with Bladder Cancer among a Chinese Han Population in Kaohsiung City

Table 2

Primers utilized in this study.

SNP	Primers, 5′-3′	Product; Size (bp)	Function
rs2165870; (A/G)	F: TCCTAGGGCTCTGAAGAATTA	383	PCR
	R: TTCAATTGACATTGCTCAGA
	FA: TGCATCTGTTTACAGCCTTA	306	Genotyping
	FG: TGCATCTGTTTACAGCCTTG
rs10802789; (C/T)	F: CTCGAAGTTAGGCGAGATTT	397	PCR
	R: GTGTGACACGTGTAGGTCAA
	FC: AAAGTGGCATTTCTCTACGC	213	Genotyping
	FT: AAAGTGGCATTTCTCTACGT
rs685550; (C/T)	F: CCACATCAAACGTCGAGAGA	348	PCR
	R: TTGAATCTATGCGCTTGTCG
	FC: ATATAGAGATATTAAACAGC	241	Genotyping
	FT: ATATAGAGATATTAAACAGT
rs7520974; (A/G)	F: TCTCCAAATCAACACTCCTG	302	PCR
	R: CATGTCCTGTAGCACCATTT
	FA: GCTGAAAGAAAGACAAATCA	175	Genotyping
	FG: GCTGAAAGAAAGACAAATCG
rs3738435; (C/T)	F: CAGTGCATGCTACCAATTAT	361	PCR
	R: TGTAGCTGACGAAATGAGTG
	FC: TAAAATAAGAGAATGAACGC	289	Genotyping
	FT: TAAAATAAGAGAATGAACGT
CHRM3RT	F: ACCCAGCTCCGAGCAGATGGAC	339	RT-qPCR
	R: CGGCTGACTCTAGCTGGATGGG
	Probe: TGCCTGAGGAGGAGCTGCTGGGGA; TGGTGGACTTGGAGAG

SNP: single-nucleotide polymorphism; F: forward; R: reverse.