Extracellular Ribonuclease from Bacillus licheniformis (Balifase), a New Member of the N1/T1 RNase Superfamily

Figure 4

Electrophoretic analysis of purity, enzymatic activity, and antibody specificity of balifase. (a) SDS/PAGE of balifase samples at different stages of purification. 1: before purification in culture fluid, 2: after chromatography on DEAE-cellulose, 3: after chromatography on phosphocellulose P-11, 4: after chromatography on UNOS₆ column, 5: binase. (b) Zymography analysis of balifase sample. 1: after chromatography on phosphocellulose P-11; 2: after chromatography on UNOS₆ column; 3: binase. (c) Zymography analysis of balifase sample after concentrating, freezing, and storage. 1: balifase; 2: binase. (d) Western Blot analysis of balifase after chromatography on UNOS₆ column. 1: binase; 2: balifase.

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