Extracellular Ribonuclease from Bacillus licheniformis (Balifase), a New Member of the N1/T1 RNase Superfamily

Table 1

Physicochemical properties of balifase as compared with binase and barnase.

RNase/characteristics	Balifase	Binase	Barnase
Number of amino acids	109	109	110
Molecular weight	12421.9	12211.6	12382.7
Theoretical pI	8.91	9.52	8.88
Extinction coefficient	19940	26930	26930
Abs 0.1% (=1 g/L)	1.605	2.205	2.175
Grand average of hydropathicity	−0.666	−0.416	−0.643
Instability index	8.92	27.25	24.27
Aliphatic index	72.48	78.81	71.00