Extracellular Ribonuclease from Bacillus licheniformis (Balifase), a New Member of the N1/T1 RNase Superfamily

Table 3

The isolation and purification of B. licheniformis RNase.

Stage of purification	Vol (V), mL	A280, units	Specific activity, units/A280	Degree of purification	Yield (by activity), %
Culture fluid after 24 h of cultivation	1200	8	763	1	100
After DEAE-cellulose, pH 5.0	1200	7.8	780	1.02	99.7
After elution from phosphocellulose in 200 mM sodium phosphate buffer, pH 7.0	20	0.7	225000	353	75
After chromatography on UNOS6 column using FPLC system	4	0.45	1.5 × 10^6	2353	50