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BioMed Research International
Volume 2016, Article ID 4275904, 8 pages
http://dx.doi.org/10.1155/2016/4275904
Research Article

Functional Characterization of 9-/13-LOXs in Rice and Silencing Their Expressions to Improve Grain Qualities

1Department of Horticulture, Washington State University, Pullman, WA 99164, USA
2State Key Lab of Rice Biology, International Atomic Energy Agency Collaborating Center, Zhejiang University, Hangzhou, Zhejiang 310029, China
3Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China
4Agricultural Extension Extending Stations, Shaoxing & Zhuji Agricultural Bureau, Shaoxing, Zhejiang 312000, China
5Institute for Wheat Research, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China

Received 5 December 2015; Accepted 24 April 2016

Academic Editor: Sudhir Sopory

Copyright © 2016 Moytri RoyChowdhury et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. J. Loiseau, B. L. Vu, M.-H. Macherel, and Y. Le Deunff, “Seed lipoxygenases: occurrence and functions,” Seed Science Research, vol. 11, no. 3, pp. 199–211, 2001. View at Google Scholar · View at Scopus
  2. D. S. Robinson, Z. Wu, C. Domoney, and R. Casey, “Lipoxygenases and the quality of foods,” Food Chemistry, vol. 54, no. 1, pp. 33–43, 1995. View at Publisher · View at Google Scholar · View at Scopus
  3. B. S. Shastry and M. R. R. Rao, “Studies on lipoxygenase from rice bran,” Cereal Chemistry, vol. 52, pp. 597–603, 1975. View at Google Scholar
  4. S. Chikubu, “Stale flavor of stored rice,” Jarq-Japan Agricultural Research Quarterly, vol. 5, pp. 63–68, 1970. View at Google Scholar
  5. Q. Long, W. Zhang, P. Wang et al., “Molecular genetic characterization of rice seed lipoxygenase 3 and assessment of its effects on seed longevity,” Journal of Plant Biology, vol. 56, no. 4, pp. 232–242, 2013. View at Publisher · View at Google Scholar · View at Scopus
  6. A. V. Torres-Penaranda, C. A. Reitmeier, L. A. Wilson, W. R. Fehr, and J. M. Narvel, “Sensory characteristics of soymilk and tofu made from lipoxygenase-free and normal soybeans,” Journal of Food Science, vol. 63, no. 6, pp. 1084–1087, 1998. View at Google Scholar · View at Scopus
  7. D. R. Iassonova, L. A. Johnson, E. G. Hammond, and S. E. Beattie, “Evidence of an enzymatic source of off flavors in ‘lipoxygenase- null’ soybeans,” Journal of the American Oil Chemists' Society, vol. 86, no. 1, pp. 59–64, 2009. View at Publisher · View at Google Scholar · View at Scopus
  8. A. V. Torres-Penaranda and C. A. Reitmeier, “Sensory descriptive analysis of soymilk,” Journal of Food Science, vol. 66, no. 2, pp. 352–356, 2001. View at Publisher · View at Google Scholar · View at Scopus
  9. M. Oozeki, T. Nagamine, T. M. Ikeda et al., “Genetic variation in lipoxygenase activity among Japanese malting barley cultivars and identification of a new lipoxygenase-1 deficient mutant,” Breeding Research, vol. 9, no. 2, pp. 55–61, 2007. View at Publisher · View at Google Scholar
  10. H. Ye, S. Harasymow, X.-Q. Zhang et al., “Sequence variation and haplotypes of lipoxygenase gene LOX-1 in the Australian barley varieties,” BMC Genetics, vol. 15, article 36, 2014. View at Publisher · View at Google Scholar · View at Scopus
  11. Y. Suzuki, I. Kazuo, C. Li, I. Honda, Y. Iwai, and U. Matsukura, “Volatile components in stored rice [Oryza sativa (L.)] of varieties with and without lipoxygenase-3 in seeds,” Journal of Agricultural and Food Chemistry, vol. 47, no. 3, pp. 1119–1124, 1999. View at Publisher · View at Google Scholar · View at Scopus
  12. I. Feussner and C. Wasternack, “The lipoxygenase pathway,” Annual Review of Plant Biology, vol. 53, pp. 275–297, 2002. View at Publisher · View at Google Scholar · View at Scopus
  13. K. Mizuno, T. Iida, A. Takano, M. Yokoyama, and T. Fujimura, “A new 9-lipoxygenase cDNA from developing rice seeds,” Plant and Cell Physiology, vol. 44, no. 11, pp. 1168–1175, 2003. View at Publisher · View at Google Scholar · View at Scopus
  14. S. Ida, Y. Masaki, and Y. Morita, “The isolation of multiple forms and product specificity of rice lipoxygenase,” Agricultural and Biological Chemistry, vol. 47, no. 3, pp. 637–641, 2014. View at Publisher · View at Google Scholar
  15. H. Ohta, S. Ida, B. Mikami, and Y. Morita, “Purification and characterization of rice lipoxygenase component 3 from rice,” Agricultural and Biological Chemistry, vol. 50, pp. 3165–3171, 1986. View at Google Scholar
  16. H. Ohta, Y. Shirano, K. Tanaka, Y. Morita, and D. Shibata, “cDNA cloning of rice lipoxygenase L-2 and characterization using an active enzyme expressed from the cDNA in Escherichia coli,” European Journal of Biochemistry, vol. 206, no. 2, pp. 331–336, 1992. View at Publisher · View at Google Scholar · View at Scopus
  17. Y.-L. Peng, Y. Shirano, H. Ohta, T. Hibino, K. Tanaka, and D. Shibata, “A novel lipoxygenase from rice: Primary structure and specific expression upon incompatible infection with rice blast fungus,” Journal of Biological Chemistry, vol. 269, no. 5, pp. 3755–3761, 1994. View at Google Scholar · View at Scopus
  18. Z. He, Study on improving the storable character of rice grains by using genetic engineering [Ph.D. dissertation], Zhejiang University, 2004.
  19. Y. Suzuki and U. Matsukura, “Lipoxygenase activity in maturing and germinating rice seeds with and without lipoxygenase-3 in mature seeds,” Plant Science, vol. 125, no. 2, pp. 119–126, 1997. View at Publisher · View at Google Scholar · View at Scopus
  20. G. Tang, G. Galili, and X. Zhuang, “RNAi and microRNA: breakthrough technologies for the improvement of plant nutritional value and metabolic engineering,” Metabolomics, vol. 3, no. 3, pp. 357–369, 2007. View at Publisher · View at Google Scholar · View at Scopus
  21. D. Miki and K. Shimamoto, “Simple RNAi vectors for stable and transient suppression of gene function in rice,” Plant and Cell Physiology, vol. 45, no. 4, pp. 490–495, 2004. View at Publisher · View at Google Scholar · View at Scopus
  22. S. Y. Dityatkin, K. V. Lisovskaya, N. N. Panzhava, and B. N. Iliashenko, “Frozen-thawed bacteria as recipients of isolated coliphage DNA,” Biochimica et Biophysica Acta (BBA)—Nucleic Acids and Protein Synthesis, vol. 281, no. 3, pp. 319–323, 1972. View at Publisher · View at Google Scholar · View at Scopus
  23. Y. Hiei, T. Komari, and T. Kubo, “Transformation of rice mediated by Agrobacterium tumefaciens,” Plant Molecular Biology, vol. 35, no. 1-2, pp. 205–218, 1997. View at Publisher · View at Google Scholar · View at Scopus
  24. S. Toki, “Rapid and efficient Agrobacterium-mediated transformation in rice,” Plant Molecular Biology Reporter, vol. 15, no. 1, pp. 16–21, 1997. View at Publisher · View at Google Scholar · View at Scopus
  25. M. RoyChowdhury, J. A. Dabul, and P. G. C. Hubstenberger, “Biotechnology advances for Arkansas rice varieties (Oryza sativa L.),” Arkansas Academy of Science, vol. 60, pp. 113–118, 2007. View at Google Scholar
  26. P. Chomczynski and N. Sacchi, “Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction,” Analytical Biochemistry, vol. 162, no. 1, pp. 156–159, 1987. View at Publisher · View at Google Scholar · View at Scopus
  27. M. W. Pfaffl, “A new mathematical model for relative quantificationin real-time RT-PCR,” Nucleic Acids Research, vol. 29, pp. 2002–2007, 2001. View at Google Scholar
  28. M. W. Pfaffl, G. W. Horgan, and L. Dempfle, “Relative Expression Software Tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR,” Nucleic Acids Research, vol. 30, no. 9, article e36, 2002. View at Publisher · View at Google Scholar · View at Scopus
  29. R. Bryant, A. Proctor, M. Hawkridge et al., “Genetic variation and association mapping of silica concentration in rice hulls using a germplasm collection,” Genetica, vol. 139, no. 11-12, pp. 1383–1398, 2011. View at Publisher · View at Google Scholar · View at Scopus
  30. C. C. Grimm, C. Bergman, J. T. Delgado, and R. Bryant, “Screening for 2-acetyl-1-pyrroline in the headspace of rice using SPME/GC-MS,” Journal of Agricultural and Food Chemistry, vol. 49, no. 1, pp. 245–249, 2001. View at Publisher · View at Google Scholar · View at Scopus
  31. J. Gil-Humanes, F. Pistón, A. Hernando, J. B. Alvarez, P. R. Shewry, and F. Barro, “Silencing of γ-gliadins by RNA interference (RNAi) in bread wheat,” Journal of Cereal Science, vol. 48, no. 3, pp. 565–568, 2008. View at Publisher · View at Google Scholar · View at Scopus
  32. A. Regina, A. Bird, D. Topping et al., “High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 10, pp. 3546–3551, 2006. View at Publisher · View at Google Scholar · View at Scopus
  33. D. Yin, S. Deng, K. Zhan, and D. Cui, “High-oleic peanut oils produced by HpRNA-mediated gene silencing of oleate desaturase,” Plant Molecular Biology Reporter, vol. 25, no. 3-4, pp. 154–163, 2007. View at Publisher · View at Google Scholar · View at Scopus
  34. S. Liu and B. Han, “Differential expression pattern of an acidic 9/13-lipoxygenase in flower opening and senescence and in leaf response to phloem feeders in the tea plant,” BMC Plant Biology, vol. 10, article 228, pp. 1471–2229, 2010. View at Publisher · View at Google Scholar · View at Scopus
  35. T. K. Park and J. C. Polacco, “Distinct lipoxygenase species appear in the hypocotyl/radicle of germinating Soybean,” Plant Physiology, vol. 90, no. 1, pp. 285–290, 1989. View at Publisher · View at Google Scholar
  36. U. Schaffrath, F. Zabbai, and R. Dudler, “Characterization of RCI-1, a chloroplastic rice lipoxygenase whose synthesis is induced by chemical plant resistance activators,” European Journal of Biochemistry, vol. 267, no. 19, pp. 5935–5942, 2000. View at Publisher · View at Google Scholar · View at Scopus
  37. Z. Kong and D. Zhao, “The inhibiting effect of abscisic acid on fragrance of kam sweet rice,” Journal of Food and Nutrition Research, vol. 2, no. 4, pp. 148–154, 2014. View at Publisher · View at Google Scholar
  38. S. S. Marla and V. K. Singh, “LOX genes in blast fungus (Magnaporthe grisea) resistance in rice,” Functional and Integrative Genomics, vol. 12, no. 2, pp. 265–275, 2012. View at Publisher · View at Google Scholar · View at Scopus