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BioMed Research International
Volume 2016, Article ID 4540826, 9 pages
http://dx.doi.org/10.1155/2016/4540826
Research Article

Enhancing the Feasibility of Microcystis aeruginosa as a Feedstock for Bioethanol Production under the Influence of Various Factors

1Department of Biotechnology, Chonnam National University, San 96-1, Dun-Duk Dong, Yeosu, Chonnam 550-749, Republic of Korea
2Department of Food Science and Nutrition, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 550-757, Republic of Korea
3Research Center on Anti-Obesity and Health Care, Chonnam National University, San 96-1, Dun-Duk Dong, Yosu, Chonnam 550-749, Republic of Korea
4Department of Environmental Oceanography, Chonnam National University, San 96-1, Dun-Duk Dong, Yeosu, Chonnam 550-749, Republic of Korea
5Department of Refrigeration Engineering, Chonnam National University, San 96-1, Dun-Duk Dong, Yeosu, Chonnam 550-749, Republic of Korea
6Department of Biotechnology and Bioengineering, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 550-757, Republic of Korea

Received 31 March 2016; Accepted 4 May 2016

Academic Editor: Pengjun Shi

Copyright © 2016 Muhammad Imran Khan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Microcystis aeruginosa, a freshwater microalga, is capable of producing and accumulating different types of sugars in its biomass which make it a good feedstock for bioethanol production. Present study aims to investigate the effect of different factors increasing growth rate and carbohydrates productivity of M. aeruginosa. MF media (modified BG11 media) and additional ingredients such as aminolevulinic acid (2 mM), lysine (2.28 mM), alanine (1 mM), and Naphthalene acetic acid (1 mM) as cytokine promoted M. aeruginosa growth and sugar contents. Salmonella showed growth-assisting effect on M. aeruginosa. Enhanced growth rate and carbohydrates contents were observed in M. aeruginosa culture grown at 25°C under red LED light of 90 μmolm−2s−1 intensity. More greenish and carbohydrates rich M. aeruginosa biomass was prepared (final OD660 nm = 2.21 and sugar contents 10.39 mM/mL) as compared to control (maximum OD660 nm = 1.4 and sugar contents 3 mM/mL). The final algae biomass was converted to algae juice through a specific pretreatment method. The resulted algae Juice was used as a substrate in fermentation process. Highest yield of bioethanol (50 mM/mL) was detected when Brettanomyces custersainus, Saccharomyces cerevisiae, and Pichia stipitis were used in combinations for fermentation process as compared to their individual fermentation. The results indicated the influence of different factors on the growth rate and carbohydrates productivity of M. aeruginosa and its feasibility as a feedstock for fermentative ethanol production.