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BioMed Research International
Volume 2016, Article ID 4567580, 12 pages
http://dx.doi.org/10.1155/2016/4567580
Research Article

Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 Cells In Vitro and In Vivo

1Department of Traditional Chinese Medicine, Huadong Hospital, Fudan University, Shanghai 200040, China
2Department of Oncology, Baoshan Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201999, China
3Department of Respiratory, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China
4Department of Traditional Chinese Medicine, Wujing Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200241, China
5Department of Rehabilitation, The Second People Huaian Hospital, Xuzhou Medical College, Huaian 223002, China
6Department of Oncology, Huadong Hospital, Fudan University, Shanghai 200040, China
7Department of Pharmacy, Huadong Hospital, Fudan University, Shanghai 200040, China

Received 5 May 2016; Accepted 20 July 2016

Academic Editor: Pratheeshkumar Poyil

Copyright © 2016 XinGang Lu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nowadays, chemotherapy is still the main effective treatment for cancer. Herb prescriptions containing Pogostemon cablin Benth (also known as “Guang-Huo-Xiang”) have been widely used in Chinese medicine today. In our research, we found that patchouli alcohol, a compound isolated from the oil of Pogostemon cablin Benth, exerted antitumor ability against human lung cancer A549 cells ability both in vitro and in vivo. MTT assay was used to assess cell viability. Hoechst 33342 staining and TUNEL cover glass staining provided the visual evidence of apoptosis. Caspase activity measurement showed that patchouli alcohol activated caspase 9 and caspase 3 of mitochondria-mediated apoptosis. Consistently, patchouli alcohol inhibited the xenograft tumor in vivo. Further investigation of the underlying molecular mechanism showed that MAPK and EGFR pathway might contribute to the antitumor effect of patchouli alcohol. Our study proved that patchouli alcohol might be able to serve as a novel antitumor compound in the clinical treatment of lung cancer.