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BioMed Research International
Volume 2016 (2016), Article ID 4575024, 7 pages
Research Article

Production and Partial Characterization of an Alkaline Xylanase from a Novel Fungus Cladosporium oxysporum

1School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
2Department of Chemical Engineering, Beijing Forestry University, Beijing 100083, China
3Beijing Biomass Energy Technology Center, State Grid Energy Conservation Service Ltd., Beijing 100053, China

Received 9 December 2015; Revised 16 February 2016; Accepted 2 March 2016

Academic Editor: Anne Hamburger

Copyright © 2016 Guo-Qiang Guan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A new fungus Cladosporium oxysporum GQ-3 producing extracellular xylanase was isolated from decaying agricultural waste and identified based on the morphology and comparison of internal transcribed spacer (ITS) rDNA gene sequence. C. oxysporum produced maximum xylanase activity of 55.92 U/mL with wheat bran as a substrate and NH4Cl as a nitrogen source. Mg2+ improved C. oxysporum xylanase production. Partially purified xylanase exhibited maximum activity at 50°C and pH 8.0, respectively, and showed the stable activity after 2-h treatment in pH 7.0–8.5 or below 55°C. Mg2+ enhanced the xylanase activity by 2% while Cu2+ had the highest inhibition ratio of 57.9%. Furthermore, C. oxysporum xylanase was resistant to most of tested neutral and alkaline proteases. Our findings indicated that Cladosporium oxysporum GQ-3 was a novel xylanase producer, which could be used in the textile processes or paper/feed industries.