The Expression of HMGB1 in Bone Marrow MSCs Is Upregulated by Hypoxia with Regulatory Effects on the Apoptosis and Adhesion

Figure 3

Effect of HMGB1 on the apoptosis of BM-MSCs. (a) Cells with different concentration of HMGB1 (0, 10, 50, 100, and 200 ng/mL) were exposed to serum deprivation (SD) for 24 hours. Apoptotic rate was calculated by FACS analysis of Annexin V/PI staining and the representative images of apoptotic cell populations were illustrated. (b) Data was shown as mean ± SEM (). versus serum(s) group. versus the group of cells exposed to SD with 0 ng/mL HMGB1.

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