Inhibitor of DNA Binding 1 Is Induced during Kidney Ischemia-Reperfusion and Is Critical for the Induction of Hypoxia-Inducible Factor-1α

Figure 4

ID1 induction in HK-2 cells H/R model. (a) Western blot analysis of ID1 in HK-2 cells under hypoxia (H) and reoxygenation (R). Cells for reoxygenation experienced 24 hours of hypoxia. β-actin served as the loading control. A representative blot from 3 independent experiments is shown. (b) Quantitative RT-PCR was used for analysis of ID1 mRNA levels in HK-2 cells under H/R. Data are expressed as mean ± SD for ID1 levels. β-actin mRNA served as the internal control (). compared with the prehypoxia controls. ID1, inhibitor of DNA binding 1; H/R, hypoxia-reoxygenation.

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