Proliferation-Related Activity in Endothelial Cells Is Enhanced by Micropower Plasma

Figure 5

Fluorescence ROS intensity in HUVECs after the micropower plasma treatment. (a) Series of fluorescent images of ROS in the cells and representative histograms of ROS intensity from 5000 cells, measured using the flow cytometer after the plasma treatment and 15-min incubation in the MM. (b) ROS in the cells was expressed as the relative intensity. The basal value in the sample before the plasma treatment was taken as 1. The data shown were represented as mean ± SD (, 148 cells). (c) Representative fluorescent images of ROS in the cells treated with the micropower plasma and 40 μM H₂O₂ for 600 s and representative histograms of ROS intensity from 5000 cells. (d) ROS in the cells treated with the micropower plasma or 40 μM H₂O₂ for 600 s was expressed as the relative intensity. The basal value in the nontreated sample was taken as 1. The data shown were represented as mean ± SD (, 147 cells). The plasma treatment resulted in a significant increase in production of ROS in the cells ( versus control and treatment of H₂O₂).

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