Long Noncoding RNA-LET Suppresses Tumor Growth and EMT in Lung Adenocarcinoma

Figure 1

Expression of lncRNA-LET in LAC patients and cell lines. (a) Analysis of lncRNA-LET expression level in paracarcinoma tissue (P) and tumor tissue (T). Total RNA was detected by quantitative real-time PCR (qRT-PCR) and GAPDH was used as an internal control. lncRNA-LET was significantly downregulated in LAC tumor tissues compared with the paracarcinoma tissue. (b) Analysis of lncRNA-LET expression level in seven cell lines was detected by using RT-PCR. GAPDH was used as an internal control. (c) Analysis of transfection efficiency in A549 cells and H1299 cells. lncRNA-LET expression in A549 cell line after being transfected with lncRNA-LET-shRNA or NC-shRNA was determined by qRT-PCR. lncRNA-LET expression in H1299 cell line after being transfected with LV-LncRNA-LET or LV-Vector was determined by qRT-PCR. Data are presented as the mean ± SD of three independent experiments. ;  .

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