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BioMed Research International
Volume 2016, Article ID 4783841, 9 pages
Research Article

Workflow for Genome-Wide Determination of Pre-mRNA Splicing Efficiency from Yeast RNA-seq Data

Department of Cell Biology, Faculty of Science, Charles University, Viničná 7, 128 43 Prague 2, Czech Republic

Received 8 September 2016; Accepted 2 November 2016

Academic Editor: Guohua Xiao

Copyright © 2016 Martin Převorovský et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The Supplementary Material contains a table of PCR primers used in this study, and all scripts required to run the analyses of splicing efficiency presented in Figures 2 and 3. The scripts are commented and can be easily adapted for other RNA-seq datasets. An adaptation of the workflow for processing RNA-seq data from the fission yeast Schizosaccharomyces pombe is also included.

  1. Supplementary Material