Serum Amyloid a Promotes Visfatin Expression in Macrophages

Figure 3

FPR2 mediated SAA-induced Visfatin production in primary monocytes. (a) Primary monocytes were isolated and cultured and then stimulated with SAA (50 μg/mL). The expression of Visfatin was detected with western blot at protein level. (b) Primary monocytes were pretreated with FPR2 siRNA (20 nM) for 6 h and with WRW⁴ (30 μM) for 1 h before the cells were stimulated with SAA. Then, WKYMVm (100 nM, a FPR2 agonist) was also used to treat the cells for 24 h. The expression of Visfatin was detected with western blot at protein level. (c) The concentration of Visfatin in the supernatants was detected with ELISA. versus control group; versus SAA treatment group. Data shown are means ± SEM from three independent experiments in duplicate.

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