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BioMed Research International
Volume 2016, Article ID 4819327, 10 pages
Research Article

Serum Amyloid a Promotes Visfatin Expression in Macrophages

Department of Cardiology II, Weifang People’s Hospital, Weifang 261041, China

Received 8 December 2015; Revised 24 January 2016; Accepted 27 January 2016

Academic Editor: Fabrizio Montecucco

Copyright © 2016 Shixun Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Fig 1: SAA up-regulates Visfatin expression in cultured RAW264.7 cells. To further confirm the effect of SAA, we also purchased recombinant human apo-SAA1 from Sigma-Aldrich (St Louis, MO). RAW264.7 macrophages were again cultured and stimulated with SAA (50 µg/ml, Sigma, SRP4324) for 24 h. The expression of Visfatin was detected with western blot and real-time PCR respectively at protein level. ** P < 0.01 versus control group. Data shown are means ± SEM from three independent experiments in duplicate.

  1. Supplementary Material