HSP27 Inhibits Homocysteine-Induced Endothelial Apoptosis by Modulation of ROS Production and Mitochondrial Caspase-Dependent Apoptotic Pathway

Figure 3

Effect of HSP27 on Hcy-induced endothelial dysfunction and apoptosis (a). Real-time RT-PCR analysis of ET-1, ICAM-1, VCAM-1, and MCP-1 mRNA expression in HUVECs. After cells were treated with 10 mM Hcy for 24 h or were not treated, total RNA was prepared and quantified using specific primers. (b) HSP27 weakened the Hcy-mediated NO decrease in HUVECs. (c) and (d) Cells that were treated with 10 mM Hcy or were not treated were analyzed after 24 h by flow cytometry. The number represents the percentages of apoptotic cells in each condition. Data are expressed as mean ± SD of three independent experiments. Columns not sharing the same superscript letter differ significantly: compared with the Neo+Hcy group; compared with the Neo group.

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