Vitamin A Inhibits Development of Dextran Sulfate Sodium-Induced Colitis and Colon Cancer in a Mouse Model

Table 1

Antibodies and immunohistochemical examination.

Antibody	Clone	Source	Fixation	Dilution	Antigen retrieval	2nd step and colorization	Interpretation
CD11c	Hamster anti-mouse CD11c	BD Pharmingen 550283	2% paraformaldehyde, frozen sections	×10	Not applied	Streptavidin Biotin/HRP kit (Jackson ImmunoResearch, West Grove, PA)	Dendritic cells
-SMA	Monoclonal mouse anti-α-SMA	DAKO M851 (DakoCytomation, Glostrup, Denmark)	10% buffered formalin, paraffin sections	×1000	Not applied	Histofine mouse staining kit (Nichirei, Tokyo, Japan)	Smooth muscle actin
IgM	Goat polyclonal	Abcam ab98673 (Abcam, Cambridge, MA)	10% buffered formalin, paraffin sections	×500	Microwave; 5 min; × 3 times	Streptavidin Biotin/HRP kit (Jackson ImmunoResearch)	IgM^+ lymphocytes
IgG	Goat polyclonal	Abcam ab98802 (Abcam, Cambridge, MA)	10% buffered formalin, paraffin sections	×500	Microwave; 5 min; × 3 times	Streptavidin Biotin/HRP kit (Jackson ImmunoResearch)	IgG^+ lymphocytes
IgA	Goat polyclonal	Abcam ab97233 (Abcam, Cambridge, MA)	10% buffered formalin, paraffin sections	×500	Microwave; 5 min; × 3 times	Streptavidin Biotin/HRP kit (Jackson ImmunoResearch)	IgA^+ lymphocytes

α-SMA: α-smooth muscle antigen.