Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari-Treated H400 Human Oral Squamous Cell Carcinoma
Table 2
The yield (mg) obtained and cytotoxicity (IC50) for fractions of D. cinnabari and subfractions DCc and DCd.
Fractions/subfractions
Yield (mg)
IC50 ± SEM (μg/mL)
DCa
3.4
29.0 ± 0.64
DCb
3.0
>30
DCc
7.0
5.8 ± 0.37
DCd
4.5
3.1 ± 0.13
DCe
4.5
18.4 ± 0.76
DCf
3.2
>30
DCg
3.9
>30
DCc1
2.0
>30
DCc2
1.0
25.4 ± 0.38
DCc3
1.0
5.1 ± 1.40
DCc4
1.6
13.1 ± 0.47
DCc5
1.7
25.3 ± 0.18
DCc6
1.4
>30
DCc7
0.9
12.4 ± 0.26
DCc8
0.9
11.8 ± 0.23
DCc9
1.4
5.4 ± 0.16
DCc10
1.9
6.1 ± 0.09
DCc11
2.2
3.0 ± 0
DCc12
1.0
3.1 ± 0.04
DCc13
1.1
3.0 ± 0
DCc14
0.8
3.4 ± 0.07
DCc15
2.0
2.9 ± 0.07
DCc16
1.3
4.2 ± 0.16
DCd1
1.0
>30
DCd2
0.7
27.9 ± 0.63
DCd3
1.8
20.7 ± 0.77
DCd4
1.5
12.8 ± 0.12
DCd5
0.8
22.3 ± 1.16
DCd6
1.2
27.6 ± 0.57
DCd7
1.3
23.8 ± 0.33
DCd8
1.6
17.8 ± 1.62
DCd9
2.8
5.6 ± 0.12
DCd10
1.0
3.9 ± 0.94
DCd11
2.0
3.1 ± 2.36
DCd12
2.0
3.2 ± 0.06
DCd13
1.5
4.0 ± 0.56
DCd14
1.7
5.0 ± 0.12
DCd15
2.4
3.3 ± 0.08
DCd16
2.0
2.9 ± 0.07
DCd17
2.0
3.9 ± 0.18
DCd18
2.0
13.2 ± 0.13
Data are presented as mean ± SEM in triplicate. Values in bold characters are considered cytotoxically active towards H400 cells (IC50 ≤ 10 μg/mL for fractions; IC50 value ≤ 4 μg/mL for subfractions). DCa, DCb, DCg: fractions of D. cinnabari. DCc1, DCc2, DCc16: subfractions isolated from fraction DCc. DCd1, DCd2, DCd18: subfractions isolated from fraction DCd.
