Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari-Treated H400 Human Oral Squamous Cell Carcinoma
Table 5
Cell cycle analysis of H400 cells at 48 and 72 hours treated with studied compounds of D. cinnabari at concentration of IC50.
Compounds
Percentage of cells (%)
Sub-G1
G1
S
G2/M
48 hours
Negative control
2.86 ± 0.27
63.52 ± 1.42
3.91 ± 0.05
17.11 ± 0.09
DC extract
27.87 ± 0.79
35.13 ± 0.62
5.21 ± 0.10
10.47 ± 0.17
DCc
29.78 ± 0.92
24.89 ± 0.43
5.90 ± 0.22
13.21 ± 0.36
DCd
30.28 ± 0.74
33.09 ± 0.50
5.47 ± 0.06
12.51 ± 0.22
DCc15
5.16 ± 0.23
62.31 ± 1.05
4.52 ± 0.06
13.37 ± 0.12
DCd16
26.33 ± 0.44
43.40 ± 1.14
6.93 ± 0.10
12.96 ± 0.17
72 hours
Negative control
7.00 ± 2.40
88.04 ± 1.16
2.27 ± 0.60
2.66 ± 1.55
DC extract
64.2 ± 7.19
24.34 ± 2.71
7.95 ± 2.32
3.18 ± 2.38
DCc
56.45 ± 2.61
30.57 ± 7.90
8.08 ± 3.52
4.32 ± 3.40
DCd
59.8 ± 7.36
26.03 ± 3.06
10.18 ± 1.91
3.93 ± 2.48
DCc15
21.43 ± 7.59
70.69 ± 2.30
5.32 ± 3.71
2.28 ± 2.04
DCd16
68.43 ± 4.38
22.56 ± 2.52
7.46 ± 1.26
1.59 ± 0.93
The values are expressed as mean ± SEM in triplicate. Significant difference compared to negative control with through one-way ANOVA.
