FBS or BSA Inhibits EGCG Induced Cell Death through Covalent Binding and the Reduction of Intracellular ROS Production

Figure 3

EGCG binds to cellular proteins. (a) Different concentrations of EGCG were added to cell lysates from untreated cells and incubated at 37°C for 9 hrs, then subject to Western blot. The cell lysate with no treatment was used as a control. The cell lysate from EGCG-treated cells (60 μM, 9 hrs) was used as a positive control. A set of proteins, including caspase-3, PARP, and α-tubulin, became increasingly difficult to detect with increased EGCG. (b) Quantitative analyses of protein expression are presented as means ± SD (). , , compared to Ctrl group. (c) Immunofluorescence staining of α-tubulin was performed in HepG₂ cells after EGCG (60 µM) treatment for 9 hrs (scale bar: 20 µm). EGCG exposure did not cause any significant changes in the expression level of α-tubulin.

(a)

(b)

(c)