A CRISPR-Based Toolbox for Studying T Cell Signal Transduction

Figure 3

A Jurkat T cell line with sunCas9 system. (a) In the CRISPRa system, dCas9 fused to SunTag is able to recruit multiple copies of VP64 and can activate gene transcription. (b-c) sgRNA expressions in JS19 cells can substantially upregulate their targeting gene expressions. JS19 cells were transduced with virus expressing the (blue histograms), the (red histogram in (b)), or the (red histograms in (c)). Cells were grown for more than a week and then analyzed for CXCR4 expression (b) and PDGFRB expression (c) in the transduced cells. The grey histograms represent the negative control (unstained sample). Data are shown in histograms and are representative of three independent experiments.

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