Research Article
A CRISPR-Based Toolbox for Studying T Cell Signal Transduction
Table 1
Comparisons between JX17, JK28, and JS19.
| ā | JX17 | JK28 | JS19 |
| Genetic screen type | Loss-of-function | Loss-of-function | Gain-of-function | Mechanism | Frame shift genome mutation | Transcriptional repression | Transcriptional activation | Regulation | Permanent | Reversible | Reversible | Target region | Gene | Gene, microRNA, and lincRNA | Gene, microRNA, and lincRNA | Efficiency | >70% | >80% | 3ā6-fold | sgRNA expression time required | >5 days | >6 days | A week | Available genome-wide libraries | Ref. [19, 20] | Ref. [10] | Ref. [10] |
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Based on [6].
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