A CRISPR-Based Toolbox for Studying T Cell Signal Transduction

Table 1

Comparisons between JX17, JK28, and JS19.

	JX17	JK28	JS19
Genetic screen type	Loss-of-function	Loss-of-function	Gain-of-function
Mechanism	Frame shift genome mutation	Transcriptional repression	Transcriptional activation
Regulation	Permanent	Reversible	Reversible
Target region	Gene	Gene, microRNA, and lincRNA	Gene, microRNA, and lincRNA
Efficiency	>70%	>80%	3–6-fold
sgRNA expression time required	>5 days	>6 days	A week
Available genome-wide libraries	Ref. [19, 20]	Ref. [10]	Ref. [10]

Based on [6].