Research Article
Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection
Table 5
Comparison of four cut-off
values in real-time PCR to fragment analysis for detection of high risk HPV (
).
| HPV type | Cut-off | Number of specimens with results | % Sensitivity | % Specificity | TP | FP | TN | FN |
| 16 | 31 | 54 | 2 | 671 | 36 | 60.0 | 99.7 | 32 | 59 | 2 | 671 | 31 | 65.6 | 99.7 | 33 | 62 | 2 | 671 | 28 | 68.9 | 99.7 | 34 | 65 | 6 | 667 | 25 | 72.2 | 99.1 |
| 18 | 31 | 31 | 7 | 721 | 4 | 88.6 | 99.0 | 32 | 32 | 9 | 713 | 3 | 91.4 | 98.8 | 33 | 33 | 10 | 718 | 2 | 94.3 | 98.6 | 34 | 33 | 12 | 716 | 2 | 94.3 | 98.4 |
| HRC | 31 | 369 | 49 | 218 | 127 | 74.4 | 81.6 | 32 | 403 | 62 | 205 | 93 | 81.3 | 76.8 | 33 | 424 | 81 | 186 | 72 | 85.5 | 69.7 | 34 | 447 | 107 | 160 | 49 | 90.1 | 59.9 |
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High risk common. True positive. False positive. True negative. False negative.
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