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BioMed Research International
Volume 2016, Article ID 5214561, 13 pages
http://dx.doi.org/10.1155/2016/5214561
Research Article

Shrimp Protein Hydrolysate Modulates the Timing of Proinflammatory Macrophages in Bupivacaine-Injured Skeletal Muscles in Rats

1School of Nutrition, Laval University, Quebec City, QC, Canada G1V 0A6
2Institute of Nutraceuticals and Functional Foods, Laval University, Quebec City, QC, Canada G1V 0A6
3Merinov, Quebec Fisheries and Aquaculture Innovation Centre, 96 montée de Sandy Beach, Office 1.07, Gaspé, QC, Canada G4X 2V6
4ISMER, Université du Québec à Rimouski, 300 allée des Ursulines, P.O. Box 3300, Rimouski, QC, Canada G5L 3A1
5Quebec Agrifood Development Center, La Pocatière, QC, Canada

Received 7 July 2016; Revised 14 September 2016; Accepted 28 September 2016

Academic Editor: Cristina Martínez-Villaluenga

Copyright © 2016 Junio Dort et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was designed to determine whether marine-derived proteins other than cod could have beneficial effects on inflammation following muscle injury. Macrophage and neutrophil densities were measured from bupivacaine-injured tibialis anterior muscle of rats fed isoenergetic diets containing either shrimp hydrolysate (Shr), casein hydrolysate (CaH), or whole casein (Ca). In this study, Shr reduced ED1+-macrophages at day 2 (), day 5 (), and day 14 after injury () compared with Ca, indicating faster resolution of inflammation in Shr. Except for day 2 after injury where Shr led to lower ED1+-macrophages compared with CaH (), both Shr and CaH responded similarly at days 5, 14, and 28 after injury. This findings suggest that beneficial effects of Shr on ED1+-cells might be related to generation of anti-inflammatory peptides through the hydrolysis process, in addition to its high content of anti-inflammatory amino acids. However, while increasing myofiber cross-sectional area in noninjured muscles compared with both Ca and CaH, Shr failed to have a positive effect in corresponding injured muscles. These data indicate that shrimp hydrolysate can facilitate resolution of inflammation after muscle injury mainly through modulating proinflammatory macrophage accumulation but have less effect on optimal recovery in terms of muscle mass and fiber size.