miR-145a-5p Promotes Myoblast Differentiation

Table 1

The primer sequences used for qRT-PCR. F: forward; R: reverse.

Gene	Primer sequence (5′→3′)	TM °C
MyoD	F: AGACTTCTATGATGACCCGTGTT	58
	R: TCAGCGTTGGTGGTCTTGC
MyoG	F: GCCCAGTGAATGCAACTCCCACA	58
	R: CAGCCGCGAGCAAATGATCTCCT
myf5	F: GAGCTGCTGAGGGAACAGGTGGAGA	58
	R: GTTCTTTCGGGACCAGACAGGGCTG
MRF4	F: ATTCTTGAGGGTGCGGATTTCCTG	58
	R: AAGACTGCTGGAGGCTGAGGCATC
ACTA1	F: CCTTTATCGGTATGGAGTCTGCG	58
	R: CCTGACATGACGTTGTTGGCA
Wnt5a	F: TACATTGGAGAAGGTGCGAAGA	58
	R: TCTCTCGGCTGCCTATTTGC
LRP5	F: CAGCACCACAAGCCACCAA	58
	R: TCCCTTCATACGAGGACACAGC
Axin2	F: AGTCCCTCCTTACCGCATGG	58
	R: AGCAGGTTCCACAGGCGTCA
β-catenin	F: TGCTGGTGACAGGGAAGACATC	58
	R: GATGGTGGGTGCAGGAGTTTAA
miR-145a-5p	GUCCAGUUUUCCCAGGAAUCCCU	61
Beta-actin	F: CAGCCTTCCTTCTTGGGTAT	58
	R: TGGCATAGAGGTCTTTACGG
U6	F: CTCGCTTCGGCAGCACA	58
	R: AACGCTTCACGAATTTGCGT