Aryl Hydrocarbon Receptor Activation by TCDD Modulates Expression of Extracellular Matrix Remodeling Genes during Experimental Liver Fibrosis

Table 1

qRT-PCR primers and annealing temperatures used in this study.

Gene	Primer sequence	Annealing temp. (°C)
Col1a1	FWD: GTCCCTGAAGTCAGCTGCATA	60
	REV: TGGGACAGTCCAGTTCTTCAT
Col3a1	FWD: CCTGGTGGAAAGGGTGAAAT	62
	REV: CGTGTTCCGGGTATACCATTAG
Col4a3	FWD: TCCTGGGGAAATGGGAAAGC	64
	REV: CTGCCTACGGATGGTTCTCC
Col4a5	FWD: TGCTCCTGAGAGATCGGCTT	58
	REV: GTTATGCTGGTGCACTTGGG
Col6a1	FWD: TCCCACCCACACAGAACAAC	58
	REV: CACTGAGAGGTGTCGTGTCC
Col6a2	FWD: TGACGCTGTTCTCTGACCTG	58
	REV: TTGTGGAAGTTCTGCTCGCC
Col6a3	FWD: CTGATGGCACCTCTCAGGAC	58
	REV: GTCACTTCCAACATCGAGGC
Dcn	FWD: AAGGGGGCCGATAAAGTTTC	58
	REV: CTGGGTTGAAAACCTCCTGC
Lox	FWD: CTGCACACACACAGGGATTG	56
	REV: AGCTGGGGTTTACACTGACC
Mmp2	FWD: ACCCAGATGTGGCCAACTAC	63
	REV: TACTTTTAAGGCCCGAGCAA
Mmp3	FWD: GTCCTCCACAGACTTGTCCC	65
	REV: GGGAGTTCCATAGAGGGACTGA
Mmp8	FWD: TACAGGGAACCCAGCACCTA	64
	REV: GGGGTTGTCTGAAGGTCCATAG
Mmp9	FWD: AAGGCAGCGTTAGCCAGAAG	63
	REV: GCGGTACAAGTATGCCTCTGC
Mmp13	FWD: GCCCTGGGAAGGAGAGACTCCAGG	55
	REV: GGATTCCCGCAAGAGTCGCAGG
Mmp14	FWD: GCCCTCTGTCCCAGATAAGC	58
	REV: ACCATCGCTCCTTGAAGACA
Plat	FWD: CAGAGATGAGCCAACGCAGA	58
	REV: TTCGCTGCAACTTCGGACAG
Plau	FWD: CATCCAGTCCTTGCGTGTCT	62
	REV: CCAAGTACACTGCCACCTTCA
Plg	FWD: ACTCAAGGGACTTTCGGTGC	58
	REV: TCAGATACTCGACGCGGTTG
Serpine1	FWD: TTCAGCCCTTGCTTGCCTC	60
	REV: ACACTTTACTCCGAAGTCGGT
Serpinh1	FWD: GGGAACGGATCGCTCCAAA	67
	REV: GGACCTGTGAGGGTTTACCAG
Timp1	FWD: CACGGGCCGCCTAAGGAACG	60
	REV: GGTCATCGGGCCCCAAGGGA
Timp2	FWD: GCCAAAGCAGTGAGCGAGAAG	56
	REV: CACACTGCTGAAGAGGGGGC
Timp3	FWD: AAGAAAAGAGCGGCAGTCCC	60
	REV: TTTGGCCCGGATCACGATG
Timp4	FWD: TATGGTAGGTGGGCTGACTGT	64
	REV: AGTTGAGACAGTGGGAGTAGGA