Altered Clock and Lipid Metabolism-Related Genes in Atherosclerotic Mice Kept with Abnormal Lighting Condition

Figure 4

Circadian expression of clock genes in fat of C57, ApoE-KO, and ApoE-KO LD/DL mice. We obtained the epididymal fat tissues of mice at time points of ZT0, ZT4, ZT8, ZT12, ZT16, and ZT20. Levels of mRNA were determined by quantitative real-time PCR. In the epididymal fat tissue, circadian rhythm was lost in Bmal1 of ApoE-KO LD/DL mice and Cry1 of both ApoE-KO and ApoE-KO LD mice. The mesors were reduced of those four clock genes in ApoE-KO LD/DL mice compared with C57 mice. The amplitude of Per2 in ApoE-KO LD/DL mice was significantly decreased compared with C57 and ApoE-KO LD/DL mice. The acrophases of Rev-erbα were delayed in ApoE-KO LD/DL mice compared with those in C57. The mRNA levels of clock genes were normalized to GAPDH mRNA ( for each group at every time point).