Altered Clock and Lipid Metabolism-Related Genes in Atherosclerotic Mice Kept with Abnormal Lighting Condition

Figure 6

Circadian expression of PPAR α, PPAR γ, ROR α, and RXR α in mouse fat tissues. We also identified the diurnal expression of lipid metabolism-associated genes (PPAR α, PPAR γ, ROR α, and RXR α) in fat tissue at specific time points. Levels of mRNA were determined by quantitative real-time PCR. In the fat tissue, PPAR γ in ApoE-KO LD/DL mice and RXR α in C57 mice exhibited circadian oscillation not in accordance with 24 hours, and PPAR γ in ApoE-KO mice, ROR α in C57 and ApoE-KO mice, and RXR α in ApoE-KO mice did not well fit the cosine curves. The mesor of PPARα was enhanced in ApoE-KO LD/DL mice compared with those in C57 and ApoE-KO mice. The mRNA levels of clock genes were normalized to GAPDH mRNA ( for each group at every time point).